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地址：北京市海淀区信息路甲28号科实大厦B座11D-05
电话：13701009079
手机：13701009079
传真：13701009079
Mail：warnerset@126.com |
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| 产品名称：PCR仪 Mastercycler® ep realplex |
| 产品品牌：德国艾本德 |
| 产品类别：PCR仪 |
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| 产品详细描述： |
产品概览：
小巧、快速、灵活 — Mastercycler ep realplex实时荧光定量PCR仪满足当前所有定量PCR应用的要求。快速温度变化速率、极短的检测时间以及直观的软件分析，从多方面压缩了实验时间，在保障准确与可靠结果的同时，每天可以完成更多的实验。该系统的灵活性更是毋庸置疑，完全开放的系统给您充分的自由使用您选择的反应管、反应板和试剂。目前，尚无任何一家实时荧光定量PCR仪可以提供如此自由的选择。
实时荧光定量PCR仪Mastercycler ep realplex紧凑的尺寸适合任何实验室，无论您的台面空间如何有限。功能模块化设计非常有利于仪器升级，例如，您可以随时将 Mastercycler® ep gradient S银质梯度PCR仪升级为实时荧光定量PCR系统Mastercycler ep realplex，当需要操作多重PCR时，也可以通过将 realplex 2模块替换为realplex 4模块而实现。
新颖的热样品保护技术防止热盖加热引起的样品预先反应，减少了由此引起的非特异PCR产物。此外，坚固的结构使用最少的可移动组件，保障安静可靠的操作，有利于实时PCR实验的结果一致性与高灵敏度。
产品特性：
Quality in all forms.
Quality in all forms.
The new optical system of the Mastercycler ep realplex offers a new proprietary particle protection technology* and very sensitve photomultipliers of the latest generation.
Automated Real-time PCR
Automated Real-time PCR
Mastercycler ep realplex & epMotion 5070 automated pipetting - Automated real time PCR for everyone!
Mastercycler ep realplex & epMotion 5070 automated pipetting - Automated real time PCR for everyone!
Real time PCR results depend pivotal on the quality of pipetting. qPCR expert S.A. Bustin demonstrated the operators influence when manually pipetting real time PCR. In his experiments the replicate Cts obtained by three individuals vaired between 8•7×105 and 2•7×103 copy numbers/µg total RNA.
(Journal of Molecular Endocrinology 29/2002). He concludes these "results provide a convincing argument for the use of robots when reproducibility and inter-laboratory comparability are the main concerns".
Real quantitative PCR needs automated pipetting
Automated epMotion pipetting can show a significant reproducibility advantage, when compared to manual set-ups. With a pipetting range down to just 1µl, its unmatched accuracy, reproducibility and easy to use philosophy mean the epMotion is the unique pipetting system for qPCR.
epMotion automated qPCR set-up guarantee highly consistent and reproducible results within and across experiments. Reactions can be performed in lower volumes and with fewer replicates, thereby reducing reagent cost significantly.
eal time PCR needs real speed
The Mastercycler ep realplex is the perfect partner for automated real time PCR. Powered by the highly accurate and uniform Mastercycler ep thermal engine, the realplex defines a new dimension of speed for automated real time PCR. Utilising the high speed silver block and impulse PCR technology, amplification can be performed in less than 30 minutes.
Packaged in a compact and robust design matched with a peace of mind real time-PCR licence ensure the Mastercycler ep realplex is a real marvel in space and time.
Mastercycler ep realplex
Real-time PCR in less than 30 minutes
User-friendly software
Highly sensitive optical system
epMotion 5070 automated pipetting system
Easy operation via control panel or PC
Preinstalled, validated applications and labware
High-precision pipetting from 1 µl to 1,000 µl
The real team
Real intuitive software and affordable packages: epMotion 5070 and Mastercycler ep realplex make automation of real time PCR available to everyone. It was never so easy to become state of the art.
Optical highlight
Optical highlight
The fluorescent dyes chosen for each experiment are excited by an array of 96 individual LEDs, which have a substantially longer lifespan in contrast to halogen lamps.
Programmed for success
Programmed for success
With a total of six different evaluation modules realplex software offers maximum flexibility for data processing
Real-time PCR Licence
Real-time PCR Licence
It is and always has been Eppendorfs policy to respect intellectual property of other parties.
技术参数：
光学模块
激发光源 96 个独立激发的发光二极管（470 nm）
检测波长 520 / 550 nm （realplex 2）
520 / 550 / 580 / 605 nm （realplex4）
检测器 1 个通道式光电倍增管（ realplex2）
2 个通道式光电倍增管（realplex4）
线性范围 9 个数量级
检测灵敏度 ≤50 fM 荧光素，可检测到单拷贝样品
加热模块
样品容量 96×0.2ml PCR 管或1 块 96 孔PCR 板
( 无裙边，半高裙边，带裙边- 按SBS 标准)
模块温控范围 4℃ - 99℃
梯度范围 1℃ -20℃（ 铝制模块）
1℃ -24℃（ 银制模块）
梯度温控范围 30℃ - 99℃
热盖温度 105℃
温度均一性 35℃ ± 0.3℃
90℃ ± 0.4℃
温控精确度 ± 0.2℃
升温速率 * 4℃ / 秒（铝制模块）
6℃ / 秒（银制模块）
降温速率 * 3℃ / 秒（铝制模块）
4.5℃ / 秒（银制模块）
全套系统
尺寸（长× 宽× 高） 26cm x 41cm x 39.6cm
重量 24 kg
加热模块重量 17 kg
检测模块重量 7 kg
电源 200-240V, 50 Hz - 60 Hz
功耗 800 W
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